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RC3H1 antibody

Overexpression
Anti-RC3H1 antibody used in Western Blot (WB). GTX129780
Anti-RC3H1 antibody used in Western Blot (WB). GTX129780

Cat No. GTX129780

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all RC3H1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , 3xFlag-human RC3H1 transfected 293T

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RC3H1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886087

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ring finger and CCCH-type domains 1 , RNF198 , ROQUIN

Cellular Localization

Cytoplasm

Background

RC3H1, or roquin, encodes a highly conserved member of the RING type ubiquitin ligase protein family (Vinuesa et al., 2005 [PubMed 15917799]). The roquin protein is distinguished by the presence of a CCCH zinc finger found in RNA-binding proteins, and localization to cytosolic RNA granules implicated in regulating mRNA translation and stability.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RC3H1 antibody used in Western Blot (WB). GTX129780

GTX129780 WB Image

Overexpression

RC3H1 antibody detects RC3H1 protein by western blot analysis. Non-transfected (-) and RC3H1-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RC3H1 antibody (GTX129780) diluted by 1:1000.

Anti-RC3H1 antibody used in Western Blot (WB). GTX129780

GTX129780 WB Image

RC3H1 antibody detects RC3H1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RC3H1 antibody (GTX129780) diluted by 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169