GeneTex
United States (US)

RC3H1 antibody

GTX31528_1130_IHC-P_20180221_18121410_984.jpg
GTX31528_1131_IHC-P_20180221_18121410_666.jpg
GTX31528_2713_WB_20180221_18121410_933.jpg

Cat No. GTX31528

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

Positive Control

Human Small Intestine Tissue Slide , HeLa Cell Lysate

Specificity/Sensitivity

At least three isoforms of RC3H1 are known to exist; this antibody will detect all three isoforms.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rabbit polyclonal RC3H1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RC3H1.The immunogen is located within amino acids 1040 - 1090 of RC3H1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ring Finger And Ccch-Type Domains 1,Rnf198,Roquin,Rc3H1

Background

RC3H1, or roquin, encodes a highly conserved member of the RING type ubiquitin ligase protein family (Vinuesa et al., 2005 [PubMed 15917799]). The roquin protein is distinguished by the presence of a CCCH zinc finger found in RNA-binding proteins, and localization to cytosolic RNA granules implicated in regulating mRNA translation and stability.[supplied by OMIM, Mar 2008]

Database

DATA IMAGES
GTX31528_1130_IHC-P_20180221_18121410_984.jpg

GTX31528 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31528 RC3H1 antibody.
Working concentration : 5 μg/ml

GTX31528_1131_IHC-P_20180221_18121410_666.jpg

GTX31528 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31528 RC3H1 antibody.
Working concentration : 20 μg/ml

GTX31528_2713_WB_20180221_18121410_933.jpg

GTX31528 WB Image

WB analysis of HeLa cell lysate using GTX31528 RC3H1 antibody.
Working concentration : 1 μg/ml

Package List Price ($)
$ 329