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RC3H1 antibody

Anti-RC3H1 antibody used in Western Blot (WB). GTX31528
Anti-RC3H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31528
Anti-RC3H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31528

Cat. No. GTX31528

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all RC3H1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

Positive Control

Human Small Intestine Tissue Slide , HeLa Cell Lysate

Product Note

At least three isoforms of RC3H1 are known to exist; this antibody will detect all three isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rabbit polyclonal RC3H1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RC3H1.The immunogen is located within amino acids 1040 - 1090 of RC3H1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ring finger and CCCH-type domains 1 , RNF198 , ROQUIN

Background

RC3H1, or roquin, encodes a highly conserved member of the RING type ubiquitin ligase protein family (Vinuesa et al., 2005 [PubMed 15917799]). The roquin protein is distinguished by the presence of a CCCH zinc finger found in RNA-binding proteins, and localization to cytosolic RNA granules implicated in regulating mRNA translation and stability.[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-RC3H1 antibody used in Western Blot (WB). GTX31528

GTX31528 WB Image

WB analysis of HeLa cell lysate using GTX31528 RC3H1 antibody.
Working concentration : 1 μg/ml

Anti-RC3H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31528

GTX31528 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31528 RC3H1 antibody.
Working concentration : 20 μg/ml

Anti-RC3H1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31528

GTX31528 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31528 RC3H1 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399