Sample (30 μg of whole cell lysate)
A: U87-MG
12% SDS PAGE
GTX116302 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using RCBTB1(GTX116302) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using RCBTB1(GTX116302) antibody at 1:200 dilution.

Sample (50 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX116302 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.