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RCC1 antibody

Anti-RCC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104590
Anti-RCC1 antibody used in Western Blot (WB). GTX104590
Anti-RCC1 antibody used in Western Blot (WB). GTX104590
Anti-RCC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104590

Cat. No. GTX104590

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RCC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Neuro2A , GL261 , 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RCC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951671

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulator of chromosome condensation 1 , CHC1 , RCC1-I , SNHG3-RCC1

Cellular Localization

Nucleus , Cytoplasm

Database

Research Area

DATA IMAGES

Anti-RCC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104590

GTX104590 ICC/IF Image

RCC1 antibody detects RCC1 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RCC1 protein stained by RCC1 antibody (GTX104590) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Anti-RCC1 antibody used in Western Blot (WB). GTX104590

GTX104590 WB Image

RCC1 antibody detects RCC1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
12 % SDS-PAGE
RCC1 antibody (GTX104590) dilution: 1:1000

Anti-RCC1 antibody used in Western Blot (WB). GTX104590

GTX104590 WB Image

Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
10% SDS PAGE
GTX104590 diluted at 1:1000

Anti-RCC1 antibody used in IHC (Paraffin sections) (IHC-P). GTX104590

GTX104590 IHC-P Image

Immunohistochemical analysis of paraffin-embedded KYSE170 xenograft, using RCC1(GTX104590) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Hayes LR et al. Elife 2020; 9 C9orf72 arginine-rich dipeptide repeat proteins disrupt karyopherin-mediated nuclear import.
Lindsey R. Hayes et al. bioRxiv 2019; (Epub) C9orf72 arginine-rich dipeptide repeat proteins disrupt importin β-mediated nuclear import.
Application : WB
Reactivity : Human
Lin YL et al. Int J Clin Exp Pathol 2015; 8 (11) : 14257-69 Methylation-silencing RCC1 expression is associated with tumorigenesis and depth of invasion in gastric cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169