GeneTex
United States (US)

RCC1 antibody

GTX104590_IHC_19011506_750.jpg
GTX104590_40002_20160817_IFA_19011506_648.jpg
GTX104590_WB_19011506_451.jpg
GTX104590_40002_WB_M_19011506_384.jpg

Cat No. GTX104590

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , Neuro2A , GL261

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RCC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CHC1 antibody, RCC1-I antibody, SNHG3-RCC1 antibody, RCC1 antibody, cell cycle regulatory protein antibody, guanine nucleotide-releasing protein antibody, SNHG3-RCC1 readthrough transcript antibody, regulator of chromosome condensation antibody, regulator of chromosome condensation 1 antibody

Cellular Localization

Nucleus , Cytoplasm

Database

Research Area

DATA IMAGES
GTX104590_IHC_19011506_750.jpg

GTX104590 IHC-P Image

Immunohistochemical analysis of paraffin-embedded KYSE170 xenograft, using RCC1(GTX104590) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104590_40002_20160817_IFA_19011506_648.jpg

GTX104590 ICC/IF Image

RCC1 antibody detects RCC1 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RCC1 protein stained by RCC1 antibody (GTX104590) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

GTX104590_WB_19011506_451.jpg

GTX104590 WB Image

Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
10% SDS PAGE
GTX104590 diluted at 1:1000

GTX104590_40002_WB_M_19011506_384.jpg

GTX104590 WB Image

RCC1 antibody detects RCC1 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
12 % SDS-PAGE
RCC1 antibody (GTX104590) dilution: 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169