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RDH11 antibody [1B4]

Overexpression
Anti-RDH11 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83716
Anti-RDH11 antibody [1B4] used in Western Blot (WB). GTX83716
Anti-RDH11 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83716
Anti-RDH11 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83716
Anti-RDH11 antibody [1B4] used in Western Blot (WB). GTX83716
Anti-RDH11 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83716
Anti-RDH11 antibody [1B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83716
Anti-RDH11 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83716
Anti-RDH11 antibody [1B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83716
Anti-RDH11 antibody [1B4] used in Flow cytometry (FACS). GTX83716

Cat. No. GTX83716

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B4

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($519)
See all RDH11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human RDH11(NP_057110) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

retinol dehydrogenase 11 , ARSDR1 , CGI82 , HCBP12 , MDT1 , PSDR1 , RALR1 , RDJCSS , SCALD , SDR7C1

Cellular Localization

Endoplasmic reticulum membrane

Background

Exhibits an oxidoreductive catalytic activity towards retinoids. Most efficient as an NADPH-dependent retinal reductase. Displays high activity towards 9-cis and all-trans-retinol. Also involved in the metabolism of short-chain aldehydes. No steroid dehydrogenase activity detected.

Database

Research Area

DATA IMAGES

Anti-RDH11 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83716

GTX83716 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX83716 RDH11 antibody [1B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RDH11 antibody [1B4] used in Western Blot (WB). GTX83716

GTX83716 WB Image

Overexpression

WB analysis of HEK293T cells transfected with RDH11 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83716 RDH11 antibody [1B4].
Loading : 5 ug per lane

Anti-RDH11 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83716

GTX83716 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX83716 RDH11 antibody [1B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RDH11 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83716

GTX83716 IHC-P Image

IHC-P analysis of prostate carcinoma tissue using GTX83716 RDH11 antibody [1B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RDH11 antibody [1B4] used in Western Blot (WB). GTX83716

GTX83716 WB Image

WB analysis of various cell lines using GTX83716 RDH11 antibody [1B4].
Loading : 35 ug per lane
Dilution : 1:200

Anti-RDH11 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83716

GTX83716 IHC-P Image

IHC-P analysis of prostate tissue using GTX83716 RDH11 antibody [1B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RDH11 antibody [1B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83716

GTX83716 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX83716 RDH11 antibody [1B4].

Anti-RDH11 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83716

GTX83716 IHC-P Image

IHC-P analysis of pancreas tissue using GTX83716 RDH11 antibody [1B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RDH11 antibody [1B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83716

GTX83716 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with RDH11 plasmid using GTX83716 RDH11 antibody [1B4].

Anti-RDH11 antibody [1B4] used in Flow cytometry (FACS). GTX83716

GTX83716 FACS Image

FACS analysis of HEK293T cells transfected with either RDH11 plasmid(Red) or empty vector control plasmid(Blue) using GTX83716 RDH11 antibody [1B4].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519