GeneTex
United States (US)

RED antibody [N1], N-term

GTX108398_IFA_19011507_190.jpg
GTX108398_39953_IHC_19011507_211.jpg
GTX108398_39953_WB_19011507_429.jpg

Cat No. GTX108398

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

THP-1 , HL-60

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human RED. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ik Cytokine,Csa2,Red,Rer,Ik

Background

The protein encoded by this gene was identified by its RED repeat, a stretch of repeated arginine, glutamic acid and aspartic acid residues. The protein localizes to discrete dots within the nucleus, excluding the nucleolus. Its function is unknown. This gene maps to chromosome 5; however, a pseudogene may exist on chromosome 2. [provided by RefSeq]

Database

DATA IMAGES
GTX108398_IFA_19011507_190.jpg

GTX108398 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RED(GTX108398) antibody at 1:200 dilution.

GTX108398_39953_IHC_19011507_211.jpg

GTX108398 IHC-P Image

RED antibody [N1], N-term detects RED protein at cytosol and nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: RED antibody [N1], N-term (GTX108398) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108398_39953_WB_19011507_429.jpg

GTX108398 WB Image

Sample (30 ug of whole cell lysate)
A: THP-1
B: HL-60
7.5% SDS PAGE
GTX108398 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169