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RED antibody [N1], N-term

Anti-RED antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108398
Anti-RED antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108398
Anti-RED antibody [N1], N-term used in Western Blot (WB). GTX108398

Cat. No. GTX108398

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RED products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

THP-1 , HL-60

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human RED. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950561

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IK cytokine , CSA2 , RED , RER

Background

The protein encoded by this gene was identified by its RED repeat, a stretch of repeated arginine, glutamic acid and aspartic acid residues. The protein localizes to discrete dots within the nucleus, excluding the nucleolus. Its function is unknown. This gene maps to chromosome 5; however, a pseudogene may exist on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RED antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108398

GTX108398 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RED(GTX108398) antibody at 1:200 dilution.

Anti-RED antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108398

GTX108398 IHC-P Image

RED antibody [N1], N-term detects RED protein at cytosol and nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: RED antibody [N1], N-term (GTX108398) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RED antibody [N1], N-term used in Western Blot (WB). GTX108398

GTX108398 WB Image

Sample (30 ug of whole cell lysate)
A: THP-1
B: HL-60
7.5% SDS PAGE
GTX108398 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169