GeneTex
United States (US)

RED antibody

GTX123717_40870_20150324_IFA_18121410_699.jpg
GTX123717_40870_IHC_18121410_923.jpg
GTX123717_40870_WB_18121410_123.jpg

Cat No. GTX123717

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

293T , A431 , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RED. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ik Cytokine,Csa2,Red,Rer,Ik

Background

The protein encoded by this gene was identified by its RED repeat, a stretch of repeated arginine, glutamic acid and aspartic acid residues. The protein localizes to discrete dots within the nucleus, excluding the nucleolus. Its function is unknown. This gene maps to chromosome 5; however, a pseudogene may exist on chromosome 2. [provided by RefSeq]

Database

DATA IMAGES
GTX123717_40870_20150324_IFA_18121410_699.jpg

GTX123717 ICC/IF Image

RED antibody detects RED protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RED protein stained by RED antibody (GTX123717) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX123717_40870_IHC_18121410_923.jpg

GTX123717 IHC-P Image

RED antibody detects IK protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
RED antibody (GTX123717) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX123717_40870_WB_18121410_123.jpg

GTX123717 WB Image

Sample (30 ug of whole cell lysate)

A: 293T

B: A431

C: HepG2

7.5% SDS PAGE

GTX123717 diluted at 1:3000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169