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RED antibody

Anti-RED antibody used in IHC (Paraffin sections) (IHC-P). GTX123717
Anti-RED antibody used in Western Blot (WB). GTX123717
Anti-RED antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123717

Cat. No. GTX123717

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RED products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

293T , A431 , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RED. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168777

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IK cytokine , CSA2 , RED , RER

Background

The protein encoded by this gene was identified by its RED repeat, a stretch of repeated arginine, glutamic acid and aspartic acid residues. The protein localizes to discrete dots within the nucleus, excluding the nucleolus. Its function is unknown. This gene maps to chromosome 5; however, a pseudogene may exist on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RED antibody used in IHC (Paraffin sections) (IHC-P). GTX123717

GTX123717 IHC-P Image

RED antibody detects IK protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
RED antibody (GTX123717) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RED antibody used in Western Blot (WB). GTX123717

GTX123717 WB Image

Sample (30 ug of whole cell lysate)

A: 293T

B: A431

C: HepG2

7.5% SDS PAGE

GTX123717 diluted at 1:3000

Anti-RED antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123717

GTX123717 ICC/IF Image

RED antibody detects RED protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RED protein stained by RED antibody (GTX123717) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169