GeneTex
United States (US)

REG III alpha antibody [C1C3]

GTX108275_39778_20181026_WB_M_pancreas_19011519_917.jpg
GTX108275_WB_19011507_645.jpg

Cat No. GTX108275

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

REG III alpha tansfected 293T cell
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human REG III alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Regenerating Family Member 3 Alpha,Hip,Hip/Pap,Ingap,Pap,Pap-H,Pap1,Pbcgf,Reg-Iii,Reg3,Reg3A

Cellular Localization

Secreted

Background

This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. Multiple alternatively spliced transcript variants encoding the same protein have been described for this gene but the full length nature of some transcripts is not yet known. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108275_39778_20181026_WB_M_pancreas_19011519_917.jpg

GTX108275 WB Image

Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with REG III alpha antibody [C1C3] (GTX108275) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX108275_WB_19011507_645.jpg

GTX108275 WB Image

Western Blot analysis of REG III alpha expression in transfected 293T cell line (20ug of whole cell lysate) A: Non-transfected lysate. B: REG III alpha transfected lysate. 12% SDS PAGE GTX108275 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169