Western Blot analysis of REG III alpha expression in transfected 293T cell line (20ug of whole cell lysate) A: Non-transfected lysate. B: REG III alpha transfected lysate. 12% SDS PAGE GTX108275 diluted at 1:500

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with REG III alpha antibody [C1C3] (GTX108275) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.