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REG III alpha antibody [C1C3]

Anti-REG III alpha antibody [C1C3] used in Western Blot (WB). GTX108275
Anti-REG III alpha antibody [C1C3] used in Western Blot (WB). GTX108275

Cat. No. GTX108275

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all REG III alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

REG III alpha transfected 293T cell , mouse pancreas

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human REG III alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037167

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regenerating family member 3 alpha , HIP , HIP/PAP , INGAP , PAP , PAP-H , PAP1 , PBCGF , REG-III , REG3

Cellular Localization

Secreted

Background

This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. Multiple alternatively spliced transcript variants encoding the same protein have been described for this gene but the full length nature of some transcripts is not yet known. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-REG III alpha antibody [C1C3] used in Western Blot (WB). GTX108275

GTX108275 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with REG III alpha antibody [C1C3] (GTX108275) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-REG III alpha antibody [C1C3] used in Western Blot (WB). GTX108275

GTX108275 WB Image

Western Blot analysis of REG III alpha expression in transfected 293T cell line (20ug of whole cell lysate) A: Non-transfected lysate. B: REG III alpha transfected lysate. 12% SDS PAGE GTX108275 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169