GeneTex
United States (US)

RENT1 antibody

Orthogonal
GTX10510_877_WB_19011506_466.jpg
GTX10510_878_WB_19011506_235.jpg

Cat No. GTX10510

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1
Not tested in other applications.

Calculated MW

124 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate pH 7.0 - 8.0, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen recognized by this antibody maps to a region between residue 250 and 300 of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Upf1, Rna Helicase And Atpase,Hupf1,Norf1,Rent1,Pnorf1,Smg-2,Upf1

Cellular Localization

Cytoplasm,P-body

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX10510_877_WB_19011506_466.jpg

GTX10510 WB Image

Orthogonal

WB analysis of IR treated or non-treated LCL1 and LCL2 cell lysate using GTX10510 RENT1 antibody.
Dilution : 1 μg/ml

GTX10510_878_WB_19011506_235.jpg

GTX10510 WB Image

WB analysis of HeLa and NIH-3T3 cell lysate using GTX10510 RENT1 antibody.
Dilution : 0.4 μg/ml
Loading : 50μg

Package List Price ($)
$ 299