GeneTex
United States (US)

RENT1 antibody

GTX16390_20180530_ICCIF_18121410_848.jpg
GTX16390_20180530_WB_18121410_680.jpg

Cat No. GTX16390

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

124 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide of human UPF1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Upf1, Rna Helicase And Atpase,Hupf1,Norf1,Rent1,Pnorf1,Smg-2,Upf1

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX16390_20180530_ICCIF_18121410_848.jpg

GTX16390 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX16390 RENT1 antibody.
Blue : DAPI

GTX16390_20180530_WB_18121410_680.jpg

GTX16390 WB Image

WB analysis of various samples using GTX16390 RENT1 antibody.
Dilution : 1:500
Loading : 25μg per lane

Package List Price ($)
$ 319