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RENT1 antibody

Anti-RENT1 antibody used in Western Blot (WB). GTX16390
Anti-RENT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16390
Anti-RENT1 antibody used in Immunoprecipitation (IP). GTX16390

Cat No. GTX16390

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human UPF1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

UPF1 RNA helicase and ATPase , HUPF1 , NORF1 , RENT1 , pNORF1 , smg-2

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Anti-RENT1 antibody used in Western Blot (WB). GTX16390

GTX16390 WB Image

WB analysis of various sample lysates using GTX16390 RENT1 antibody.
Dilution : 1:500
Loading : 25μg per lane

Anti-RENT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16390

GTX16390 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX16390 RENT1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-RENT1 antibody used in Immunoprecipitation (IP). GTX16390

GTX16390 IP Image

IP analysis of HeLa cell lysate using GTX16390 RENT1 antibody.
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339