U87-MG whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with REST antibody (GTX129144) diluted at 1:500.

Wild-type (WT) and REST knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with REST antibody (GTX129144) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REST antibody detects REST protein at cytoplasm and nucleus in human lung by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
REST antibody (GTX129144) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REST antibody detects REST protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
5 % SDS-PAGE
REST antibody (GTX129144) dilution: 1:1000