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REST antibody

Anti-REST antibody used in IHC (Paraffin sections) (IHC-P). GTX37363
Anti-REST antibody used in Western Blot (WB). GTX37363

Cat. No. GTX37363

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μg ($399)
See all REST products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-2000
IHC-P 1:400-800
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.01M TBS, 0.5% BSA, 25% Glycerol

Preservative

0.015% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide derived between 541-440 amino acids of human NRSF/REST

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11168666

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RE1 silencing transcription factor , DFNA27 , GINGF5 , HGF5 , NRSF , WT6 , XBR

Cellular Localization

Nucleus,Cytoplasm

Background

This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018]

Database

Research Area

DATA IMAGES

Anti-REST antibody used in IHC (Paraffin sections) (IHC-P). GTX37363

GTX37363 IHC-P Image

IHC-P analysis of human gastric tissue using GTX37363 REST antibody.

Anti-REST antibody used in Western Blot (WB). GTX37363

GTX37363 WB Image

WB analysis of 30μg of rat brain (Lane 1) and human colon carcinoma (Lane 2) tissue lysates using GTX37363 REST antibody.

REFERENCE

Application Reference

Casadei E et al. Chem Senses 2019; (Epub) Commensal bacteria regulate gene expression and differentiation in vertebrate olfactory systems through transcription factor REST.
Application : WB
Reactivity : Mouse, Zebrafish
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REVIEW

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SDS
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399