APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
4-6μg/ml |
Note :
IHC-P
Human Testis shows nuclear staining in spermatogonia and spermatocytes.
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Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX25088-PEP).
Product Note
This antibody is expected to recognise both reported isoforms.
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-VLQQTYGSTLKVT, from the C Terminus of the protein sequence according to NP_057400.1; NP_001032961.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
REV1 DNA directed polymerase , AIBP80 , REV1L
Cellular Localization
Nucleus
Background
This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]
Database
Research Area
DATA IMAGES
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GTX25088 IHC-P Image
IHC-P analysis of human testis using GTX25088 REV1 antibody, C-term. Antigen retrieval : Tris/EDTA buffer pH 9 Dilution : 4μg/ml
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REFERENCE
There are currently no references for REV1 antibody, C-term (GTX25088). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for REV1 antibody, C-term (GTX25088). Be the first to share your experience with this product.