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RFC1 antibody

Anti-RFC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129291
Anti-RFC1 antibody used in Western Blot (WB). GTX129291
Anti-RFC1 antibody used in Immunoprecipitation (IP). GTX129291

Cat. No. GTX129291

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RFC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RFC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885957

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication factor C subunit 1 , A1 , MHCBFB , PO-GA , RECC1 , RFC , RFC140

Cellular Localization

Nucleus

Background

The protein encoded by this gene is the large subunit of replication factor C, which is a five subunit DNA polymerase accessory protein. Replication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RFC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129291

GTX129291 ICC/IF Image

RFC1 antibody detects RFC1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RFC1 protein stained by RFC1 antibody (GTX129291) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RFC1 antibody used in Western Blot (WB). GTX129291

GTX129291 WB Image

RFC1 antibody detects RFC1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
RFC1 antibody (GTX129291) dilution: 1:1000

Anti-RFC1 antibody used in Immunoprecipitation (IP). GTX129291

GTX129291 IP Image

RFC1 antibody immunoprecipitates RFC1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. 35 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of RFC1 protein by 4 μg RFC1 antibody (GTX129291)
D. Immunoprecipitation of RFC1 protein by 4 μg RFC1 antibody (GTX129292)
5 % SDS-PAGE
The immunoprecipitated RFC1 protein was detected by RFC1 antibody (GTX129291) diluted at 1:1500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

Application Reference

Maltby CJ et al. bioRxiv 2023; AAGGG repeat expansions trigger RFC1-independent synaptic dysregulation in human CANVAS Neurons.
Cortese A et al. Nat Genet 2019; 51 (4) : 649-658 Biallelic expansion of an intronic repeat in RFC1 is a common cause of late-onset ataxia.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169