RFC1 antibody

Cat. No. GTX129292

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all RFC1 products

Datasheet
Document

View product citations for antibody GTX129292 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RFC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885958

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication factor C subunit 1 , A1 , MHCBFB , PO-GA , RECC1 , RFC , RFC140

Cellular Localization

Nucleus

Background

The protein encoded by this gene is the large subunit of replication factor C, which is a five subunit DNA polymerase accessory protein. Replication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability. [provided by RefSeq]

Database

Gene ID: 5981 RFC1
UniProt: P35251 RFC1

Research Area

DATA IMAGES

GTX129292 IP Image

Immunoprecipitation of RFC1 protein from HeLa whole cell extracts using 5 μg of RFC1 antibody (GTX129292).
Western blot analysis was performed using RFC1 antibody (GTX129292) diluted at 1:1500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX129292 WB Image

RFC1 antibody detects RFC1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5 % SDS-PAGE, and blotted with RFC1 antibody (GTX129292) diluted by 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty