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RFC1 antibody

Anti-RFC1 antibody used in Western Blot (WB). GTX129292
Anti-RFC1 antibody used in Immunoprecipitation (IP). GTX129292

Cat No. GTX129292

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RFC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

replication factor C subunit 1 , A1 , MHCBFB , PO-GA , RECC1 , RFC , RFC140

Cellular Localization

Nucleus

Background

The protein encoded by this gene is the large subunit of replication factor C, which is a five subunit DNA polymerase accessory protein. Replication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RFC1 antibody used in Western Blot (WB). GTX129292

GTX129292 WB Image

RFC1 antibody detects RFC1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5 % SDS-PAGE, and blotted with RFC1 antibody (GTX129292) diluted by 1:1000

Anti-RFC1 antibody used in Immunoprecipitation (IP). GTX129292

GTX129292 IP Image

Immunoprecipitation of RFC1 protein from HeLa whole cell extracts using 5 μg of RFC1 antibody (GTX129292).
Western blot analysis was performed using RFC1 antibody (GTX129292) diluted at 1:1500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

There are currently no references for RFC1 antibody (GTX129292). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RFC1 antibody (GTX129292). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169