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RFC1 antibody

Anti-RFC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55779
Anti-RFC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55779
Anti-RFC1 antibody used in Western Blot (WB). GTX55779

Cat. No. GTX55779

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all RFC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human RFC1 (NP_001191676.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication factor C subunit 1 , A1 , MHCBFB , PO-GA , RECC1 , RFC , RFC140

Cellular Localization

Nucleus

Background

This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-RFC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55779

GTX55779 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX55779 RFC1 antibody.
Blue : DAPI

Anti-RFC1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55779

GTX55779 ICC/IF Image

ICC/IF analysis of A549 cells using GTX55779 RFC1 antibody.

Anti-RFC1 antibody used in Western Blot (WB). GTX55779

GTX55779 WB Image

WB analysis of MCF7 cell lysate using GTX55779 RFC1 antibody.
Dilution : 1:500
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399