GeneTex
  • Country / Location Selection

United States (US)

RFC2 antibody

Anti-RFC2 antibody used in Western Blot (WB). GTX105087
Anti-RFC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105087
Anti-RFC2 antibody used in Western Blot (WB). GTX105087

Cat No. GTX105087

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , H1299

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RFC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

replication factor C subunit 2 , RFC40

Cellular Localization

Nucleus

Background

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-RFC2 antibody used in Western Blot (WB). GTX105087

GTX105087 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
10% SDS PAGE
RFC2 antibody
GTX105087 diluted at 1:1000

Anti-RFC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105087

GTX105087 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RFC2(GTX105087) antibody at 1:500 dilution.

Anti-RFC2 antibody used in Western Blot (WB). GTX105087

GTX105087 WB Image

RFC2 antibody detects RFC2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC2 antibody (GTX105087) diluted by 1:1000.

REFERENCE
There are currently no references for RFC2 antibody (GTX105087). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RFC2 antibody (GTX105087). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169