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RFC3 antibody

Anti-RFC3 antibody used in Western Blot (WB). GTX103706
Anti-RFC3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103706
Anti-RFC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103706

Cat. No. GTX103706

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RFC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RFC3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618018

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication factor C subunit 3 , RFC38

Cellular Localization

Nucleus

Background

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RFC3 antibody used in Western Blot (WB). GTX103706

GTX103706 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
replication factor C3 antibody
GTX103706 diluted at 1:1000

Anti-RFC3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103706

GTX103706 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RFC3(GTX103706) antibody at 1:200 dilution.

Anti-RFC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103706

GTX103706 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using RFC3(GTX103706) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169