GeneTex
United States (US)

RFC4 antibody [N1C3]

GTX104052_40079_20180518_WB_19011506_973.jpg
GTX104052_IFA_19011506_711.jpg
GTX104052_40079_20151227_IHC-P_19011506_541.jpg
GTX104052_IHC_19011506_725.jpg
GTX104052_40079_WB_M_liver_19011506_485.jpg

Cat No. GTX104052

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Mouse liver

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RFC4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Replication Factor C Subunit 4,A1,Rfc37,Rfc4

Cellular Localization

Nucleus

Background

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104052_40079_20180518_WB_19011506_973.jpg

GTX104052 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC4 antibody [N1C3] (GTX104052) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104052_IFA_19011506_711.jpg

GTX104052 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using RFC4(GTX104052) antibody at 1:200 dilution.

GTX104052_40079_20151227_IHC-P_19011506_541.jpg

GTX104052 IHC-P Image

RFC4 antibody [N1C3] detects RFC4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded MDA-MB-231xenograft.
RFC4 antibody [N1C3] (GTX104052) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104052_IHC_19011506_725.jpg

GTX104052 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using RFC4(GTX104052) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104052_40079_WB_M_liver_19011506_485.jpg

GTX104052 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX104052 diluted at 1:500

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169