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RFC4 antibody [N1C3]

Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052
Anti-RFC4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104052
Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052
Anti-RFC4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104052
Anti-RFC4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104052

Cat. No. GTX104052

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RFC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Mouse liver , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RFC4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951693

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication factor C subunit 4 , A1 , RFC37

Cellular Localization

Nucleus

Background

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052

GTX104052 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC4 antibody [N1C3] (GTX104052) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RFC4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104052

GTX104052 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using RFC4(GTX104052) antibody at 1:200 dilution.

Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052

GTX104052 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX104052 diluted at 1:500

Anti-RFC4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104052

GTX104052 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using RFC4(GTX104052) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RFC4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104052

GTX104052 IHC-P Image

RFC4 antibody [N1C3] detects RFC4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded MDA-MB-231xenograft.
RFC4 antibody [N1C3] (GTX104052) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chen BR et al. J Cell Biol 2019; 218 (7) : 2113-2123 XLF and H2AX function in series to promote replication fork stability.
Lopez-Contreras AJ et al. Cell Rep 2013; 3 (4) : 1105-16 A proteomic characterization of factors enriched at nascent DNA molecules.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169