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RFC4 antibody

Anti-RFC4 antibody used in Western Blot (WB). GTX04241
Anti-RFC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04241
Anti-RFC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04241

Cat. No. GTX04241

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($399)
See all RFC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human RFC4(Accession P35249), corresponding to amino acid residues N125-L149.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication factor C subunit 4 , A1 , RFC37

Cellular Localization

Nucleus

Background

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RFC4 antibody used in Western Blot (WB). GTX04241

GTX04241 WB Image

WB analysis of COS-7 cell lysates using GTX04241 RFC4 antibody. The lane on the left was treated with blocking peptide.

Anti-RFC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04241

GTX04241 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX04241 RFC4 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-RFC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04241

GTX04241 IHC-P Image

IHC-P analysis of human gastric cancer GTX04241 RFC4 antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399