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RFP antibody [GT1433]

Anti-RFP antibody [GT1433] used in Immunoprecipitation (IP). GTX628898
Anti-RFP antibody [GT1433] used in Western Blot (WB). GTX628898

Cat. No. GTX628898

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1433

Isotype

 IgG1

Application

 WB, IP
Package
100 μl ($399),
25 μl ($169)
See all RFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293T expressing RFP-tagged protein

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of RFP. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888074

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-RFP antibody [GT1433] used in Immunoprecipitation (IP). GTX628898

GTX628898 IP Image

RFP tag antibody immunoprecipitates RFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 μg of RFP tag antibody (GTX628898) 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody (GTX628898) diluted at 1:1000. EasyBlot anti-nouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-RFP antibody [GT1433] used in Western Blot (WB). GTX628898

GTX628898 WB Image

RFP antibody detects RFP protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T whole cell extract expressing GFP-tagged protein
C. 30 μg 293T whole cell extract expressing RFP-tagged protein
12 % SDS-PAGE
RFP antibody (GTX628898) dilution: 1:10000

REFERENCE

Application Reference

Klymenko Y et al. Neoplasia 2017; 19 (7) : 549-563 Heterogeneous Cadherin Expression and Multicellular Aggregate Dynamics in Ovarian Cancer Dissemination.
Application : WB
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations