GeneTex
  • Country / Location Selection

United States (US)

RFP antibody [GT1610]

Anti-RFP antibody [GT1610] used in Immunoprecipitation (IP). GTX628545
Anti-RFP antibody [GT1610] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628545
Anti-RFP antibody [GT1610] used in Western Blot (WB). GTX628545

Cat No. GTX628545

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1610

Isotype

IgG2a

Application

WB, ICC/IF, IP
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

RFP-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of RFP. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888048

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-RFP antibody [GT1610] used in Immunoprecipitation (IP). GTX628545

GTX628545 IP Image

RFP tag antibody immunoprecipitates RFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 μg of RFP tag antibody (GTX628545) 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody (GTX628545) diluted at 1:1000. EasyBlot anti-nouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-RFP antibody [GT1610] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628545

GTX628545 ICC/IF Image

RFP-tag antibody detects RFP-recombinant protein by immunofluorescent analysis. Sample: RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFP-recombinant protein stained by RFP-tag antibody (GTX628545) diluted at 1:2000. Red: RFP is expressed in the tranfected cell . Blue: Hoechst 33342 staining.

Anti-RFP antibody [GT1610] used in Western Blot (WB). GTX628545

GTX628545 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody [GT1610] (GTX628545) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for RFP antibody [GT1610] (GTX628545). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RFP antibody [GT1610] (GTX628545). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169