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RFP antibody [HL2028]

Recombinant antibody
Anti-RFP antibody [HL2028] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637929
Anti-RFP antibody [HL2028] used in Western Blot (WB). GTX637929

Cat. No. GTX637929

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2028

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all RFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length RFP recombinant protein

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-RFP antibody [HL2028] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637929

GTX637929 ICC/IF Image

RFP-tag antibody detects RFP tagged protein by immunofluorescent analysis.
Sample: RFP tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RFP tagged protein stained by RFP-tag antibody (GTX637929) diluted at 500.
Red: RFP tagged protein is expressed in the transfected cells.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RFP antibody [HL2028] used in Western Blot (WB). GTX637929

GTX637929 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted antibodies as follows:
Upper image: RFP antibody [HL2028], GTX637929 (1:5000)
Middle image: mCherry-tagged protein antibody, GTX128508
Lower image: FP-tagged protein antibody, GTX635070
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations