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RFP antibody [RF5R]

Anti-RFP antibody [RF5R] used in Western Blot (WB). GTX82561

Cat No. GTX82561

Host

Mouse

Clonality

Monoclonal

Clone Name

RF5R

Isotype

IgG1

Application

WB, IHC-P, IHC-Fr, IP, Dot, ELISA, Immunostaining
Package
100 μg ($339)

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-3000
Immunostaining: Use at a dilution of 1:500 - 1:2000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Calculated MW

27 kDa. ( Note )

Product Note

Recognizes native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11178232

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RFP tag , RFP , dsRed , red fluorescent protein

Background

ince the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.

Research Area

DATA IMAGES

Anti-RFP antibody [RF5R] used in Western Blot (WB). GTX82561

GTX82561 WB Image

1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with RFP-tagged protein vector; untransfected control (1), transfected with Turbo-RFP (2), and transfected with DsRed (3)

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 339