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RFP antibody [RF5R]

Anti-RFP antibody [RF5R] used in Western Blot (WB). GTX82561

Cat. No. GTX82561

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 RF5R

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, IP, Dot, ELISA, Immunostaining

Reactivity

 Species independent
Package
100 μg ($399)
See all RFP products

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-3000
Immunostaining: Use at a dilution of 1:500 - 1:2000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Calculated MW

27 kDa. ( Note )

Product Note

Recognizes native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, tdTomato, etc.

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11178232

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RFP tag , RFP , dsRed , red fluorescent protein

Background

ince the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.

Research Area

DATA IMAGES

Anti-RFP antibody [RF5R] used in Western Blot (WB). GTX82561

GTX82561 WB Image

1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with RFP-tagged protein vector; untransfected control (1), transfected with Turbo-RFP (2), and transfected with DsRed (3)

REFERENCE

Application Reference

Ellis PS et al. Immun Ageing 2022; 19 (1) : 31 A subset of gut leukocytes has telomerase-dependent "hyper-long" telomeres and require telomerase for function in zebrafish.
Bertozzi A et al. Dev Biol 2020; (Epub) Is zebrafish heart regeneration "complete"? Lineage-restricted cardiomyocytes proliferate to pre-injury numbers but some fail to differentiate in fibrotic hearts.
Application : IHC-Fr
Vega-Cabrera LA et al. PLoS One 2017; 12 (2) : e0172737 Analysis of Spo0M function in Bacillus subtilis.

Clone Reference

Jun S et al. Nat Commun 2016; 7:10994 LIG4 mediates Wnt signalling-induced radioresistance.
Application : IHC-P
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations