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RFP antibody

Anti-RFP antibody used in Immunoprecipitation (IP). GTX127897
Anti-RFP antibody used in Western Blot (WB). GTX127897
Anti-RFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127897

Cat No. GTX127897

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

RFP antibody recognizes the red fluorescent protein (RFP), a fluorophore often used in conjunction with the green fluorescent protein (GFP). Like GFP, RFP allows visualization of fusion proteins in cells and is one of the most frequently used tools to monitor in vivo biological processes. This RFP antibody does not cross-react with the GFP protein.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:5000
IP 1:10000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293T whole cell extract expressing RFP-tagged protein

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length RFP recombinant protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Red fluorescent protein

Research Area

DATA IMAGES

Anti-RFP antibody used in Immunoprecipitation (IP). GTX127897

GTX127897 IP Image

RFP antibody Immunoprecipitation of RFP-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-RFP antibody (GTX127897). (A) Input, 15ug of HeLa transfected lysate. The precipitated protein was detected by GTX127897 diluted at 1:10000.

Anti-RFP antibody used in Western Blot (WB). GTX127897

GTX127897 WB Image

RFP antibody detects RFP protein by western blot analysis.
A. 5 μg 293T whole cell extract
B. 5 μg 293T whole cell extract expressing GFP-tagged protein
C. 5 μg 293T whole cell extract expressing RFP-tagged protein
12% SDS-PAGE
RFP antibody (GTX127897) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RFP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127897

GTX127897 ICC/IF Image

RFP antibody detects RFP protein at by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RFP protein stained by RFP antibody (GTX127897) diluted at 1:5000.
Red: RFP is expressed in the RFP-fusion protein tranfected cell.
Blue: Hoechst 33342 staining.
The cell of merger panel with no RFP expressed as a negative control.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169