RFP antibody

RFP antibody Immunoprecipitation of RFP-tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-RFP antibody (GTX127897). (A) Input, 15ug of HeLa transfected lysate. The precipitated protein was detected by GTX127897 diluted at 1:10000.

RFP antibody detects RFP protein at by immunofluorescent analysis.
Sample: RFP tagged protein transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RFP protein stained by RFP antibody (GTX127897) diluted at 1:5000.
Red: RFP is expressed in the RFP-fusion protein tranfected cell.
Blue: Hoechst 33342 staining.
The cell of merger panel with no RFP expressed as a negative control.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody (GTX127897) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RFP antibody (GTX127897) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.