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RFX1 antibody

Anti-RFX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31948
Anti-RFX1 antibody used in Western Blot (WB). GTX31948
Anti-RFX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31948

Cat No. GTX31948

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

Specificity/Sensitivity

RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist; this antibody will detect both isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1.The immunogen is located within the last 50 amino acids of RFX1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

regulatory factor X1 , EFC , RFX

Cellular Localization

Nucleus

Background

This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RFX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31948

GTX31948 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31948 RFX1 antibody.
Working concentration : 5 μg/ml

Anti-RFX1 antibody used in Western Blot (WB). GTX31948

GTX31948 WB Image

WB analysis of HeLa cell lysate using GTX31948 RFX1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-RFX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31948

GTX31948 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31948 RFX1 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339