APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa Cell Slide(GTX25529) , HeLa Cell Lysate
Product Note
RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist; this antibody will detect both isoforms.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1.The immunogen is located within the last 50 amino acids of RFX1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
regulatory factor X1 , EFC , RFX
Cellular Localization
Nucleus
Background
This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31948 WB Image
WB analysis of HeLa cell lysate using GTX31948 RFX1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX31948 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX31948 RFX1 antibody.
Working concentration : 5 μg/ml
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GTX31948 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX31948 RFX1 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml
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REFERENCE
There are currently no references for RFX1 antibody (GTX31948). Be the first to share your publications with this product.
REVIEW
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