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United States (US)

RFX5 antibody

Cat. No. GTX26557

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, ChIP assay, EMSA

Reactivity

 Human
Package
100 μg ($589)
See all RFX5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ELISA 1:5000-1:25000
ChIP assay Assay dependent
EMSA Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RFX5 (C-terminal specific) peptide corresponding to a region near the C-terminus of the human protein conjugated to KLH.

Purification

IgG fraction
This product was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulatory factor X5

Cellular Localization

Nucleus

Background

RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex, which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.

Database

Research Area

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589