GeneTex
United States (US)

RFX5 antibody

GTX27542_20160330_WB_18121410_941.jpg

Cat No. GTX27542

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA, ChIP assay, GSA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ELISA 1:5,000 - 1:25,000
ChIP assay Assay dependent
GSA 0.5 μL - 1.0 μL

Note :

WB
This antibody may react with unknown minor bands at 65 kDa and 80 kDa.

Not tested in other applications.

Calculated MW

65 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Purification


This product was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Regulatory Factor X5,Rfx5

Background

RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex, which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.

Database

DATA IMAGES
GTX27542_20160330_WB_18121410_941.jpg

GTX27542 WB Image

Western Blot of Rabbit Anti-RFX5 antibody (GTX27542). Raji B cell nuclear extract lysates. Load: 35 µg per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG at 1:5,000 for 45 min at RT.

Package List Price ($)
$ 389