*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Over expressed COS-7 cell lysates (clean single band) and Raji cell nuclear extracts (demonstrating
Reacts with residues 18-31 [ASELGDPEDPGEEAC] of the RFX-B protein.
0.02% Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide made to a portion of the RFX-B protein.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
regulatory factor X associated ankyrin containing protein , ANKRA1 , BLS , F14150_1 , RFX-B
Cytoplasm , Nucleus
Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2013]
GTX30257 ELISA Image
Detection of RFX-B using GTX30257 as the capture antibody in ELISA
GTX30257 WB Image
Western blot analysis of RFX-B in Raji lymphoblastoid cell line with anti-RFX-B at 1:500 dilution.