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RFXANK antibody

Anti-RFXANK antibody used in ELISA (ELISA). GTX30257
Anti-RFXANK antibody used in Western Blot (WB). GTX30257

Cat No. GTX30257

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Primate
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Over expressed COS-7 cell lysates (clean single band) and Raji cell nuclear extracts (demonstrating

Product Note

Reacts with residues 18-31 [ASELGDPEDPGEEAC] of the RFX-B protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide made to a portion of the RFX-B protein.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

regulatory factor X associated ankyrin containing protein , ANKRA1 , BLS , F14150_1 , RFX-B

Cellular Localization

Cytoplasm , Nucleus

Background

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-RFXANK antibody used in ELISA (ELISA). GTX30257

GTX30257 ELISA Image

Detection of RFX-B using GTX30257 as the capture antibody in ELISA

Anti-RFXANK antibody used in Western Blot (WB). GTX30257

GTX30257 WB Image

Western blot analysis of RFX-B in Raji lymphoblastoid cell line with anti-RFX-B at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379