RGS1 antibody [HL1402]

Cat. No. GTX636868

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1402
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all RGS1 products

Datasheet

View product citations for antibody GTX636868 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RGS1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulator of G protein signaling 1 , 1R20 , BL34 , HEL-S-87 , IER1 , IR20

Cellular Localization

Cell membrane,Cytoplasm, cytosol

Background

This gene encodes a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5996 RGS1
UniProt: Q08116 RGS1

Research Area

DATA IMAGES

GTX636868 ICC/IF Image

RGS1 antibody [HL1402] detects RGS1 protein at cell membrane by immunofluorescent analysis.
Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RGS1 stained by RGS1 antibody [HL1402] (GTX636868) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636868 IHC-P Image

RGS1 antibody [HL1402] detects RGS1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
RGS1 stained by RGS1 antibody [HL1402] (GTX636868) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636868 WB Image

Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS1 antibody [HL1402] (GTX636868) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations