GeneTex

  • Country / Location Selection

United States (US)

RGS1 antibody

Orthogonal
Anti-RGS1 antibody used in Western Blot (WB). GTX112803
Anti-RGS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112803
Anti-RGS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112803

Cat. No. GTX112803

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RGS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RGS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178048

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulator of G protein signaling 1 , 1R20 , BL34 , HEL-S-87 , IER1 , IR20

Background

This gene encodes a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RGS1 antibody used in Western Blot (WB). GTX112803

GTX112803 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS1 antibody (GTX112803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RGS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112803

GTX112803 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RGS1(GTX112803) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RGS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112803

GTX112803 ICC/IF Image

Orthogonal

RGS1 antibody detects RGS1 protein at cell membrane by immunofluorescent analysis.
Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RGS1 stained by RGS1 antibody (GTX112803) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Kashani-Sabet M et al. Clin Cancer Res 2017; (Epub) Prospective Validation of Molecular Prognostic Markers in Cutaneous Melanoma: A Correlative Analysis of E1690.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for RGS1 antibody (GTX112803). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169