APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.5-2μg/ml |
2.5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX22715-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-NLLNDLQANSLK, from the C Terminus of the protein sequence according to NP_002913.3.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
regulator of G protein signaling 1 , 1R20 , BL34 , HEL-S-87 , IER1 , IR20
Cellular Localization
Cell membrane , Cytoplasm, cytosol
Background
This gene encodes a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX22715 IHC-P Image
IHC-P analysis of human thymus using GTX22715 RGS1 antibody. Antigen retrieval : citrate buffer pH 6 Dilution : 2.5μg/ml
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GTX22715 WB Image
WB analysis of HepG2 lysate using GTX22715 RGS1 antibody. Dilution : 1μg/ml Loading : 30μg protein in RIPA buffer
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GTX22715 IHC-P Image
IHC-P analysis of human small intestine using GTX22715 RGS1 antibody. Antigen retrieval : citrate buffer pH 6 Dilution : 2.5μg/ml
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REFERENCE
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REVIEW
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