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RGS13 antibody [N1C3]

Anti-RGS13 antibody [N1C3] used in Western Blot (WB). GTX113540
Anti-RGS13 antibody [N1C3] used in Western Blot (WB). GTX113540
Anti-RGS13 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113540

Cat. No. GTX113540

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RGS13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , human RGS13-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RGS13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037873

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulator of G protein signaling 13

Background

The protein encoded by this gene is a member of the regulator of G protein signaling (RGS) family. RGS family members share similarity with S. cerevisiae SST2 and C. elegans egl-10 proteins, which contain a characteristic conserved RGS domain. RGS proteins accelerate GTPase activity of G protein alpha-subunits, thereby driving G protein into their inactive GDP-bound form, thus negatively regulating G protein signaling. RGS proteins have been implicated in the fine tuning of a variety of cellular events in response to G protein-coupled receptor activation. The biological function of this gene, however, is unknown. Two transcript variants encoding the same isoform exist. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RGS13 antibody [N1C3] used in Western Blot (WB). GTX113540

GTX113540 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RGS13 antibody [N1C3] (GTX113540) diluted at 1:1000.

Anti-RGS13 antibody [N1C3] used in Western Blot (WB). GTX113540

GTX113540 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RGS13 antibody [N1C3] (GTX113540) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

Anti-RGS13 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113540

GTX113540 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using RGS13(GTX113540) antibody at 1:200 dilution.

REFERENCE

Application Reference

Wang JH et al. Arthritis Rheum 2013; 65 (10) : 293625 Extension of the germinal center stage of B cell development promotes autoantibodies in BXD2 mice.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169