GeneTex
United States (US)

RGS14 antibody [C3], C-term

GTX106412_39890_IHC_19011506_556.jpg
GTX106412_39890_WB_19011506_688.jpg

Cat No. GTX106412

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

Jurkat
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RGS14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RGS14 antibody, regulator of G-protein signaling 14 antibody, regulator of G-protein signalling 14 antibody

Cellular Localization

Membrane

Background

This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX106412_39890_IHC_19011506_556.jpg

GTX106412 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using RGS14(GTX106412) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106412_39890_WB_19011506_688.jpg

GTX106412 WB Image

RGS14 antibody [C3], C-term detects RGS14 protein by Western blot analysis.
A. 30 µg Jurkat whole lysate/extract
7.5 % SDS-PAGE
RGS14 antibody [C3], C-term (GTX106412) dilution: 1:500

SDS
Glycerol.pdf
Thimerosal.pdf