GeneTex
United States (US)

RGS17 antibody [C2C3], C-term

GTX106449_40150_IFA_19011506_118.jpg
GTX106449_40150_WB_B_19011506_615.jpg

Cat No. GTX106449

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

293T transfected recombinant RGS17
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RGS17. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RGS-17 antibody, RGSZ2 antibody, hRGS17 antibody, RGS17 antibody, regulator of G-protein signaling 17 antibody

Cellular Localization

Membrane; Lipid-anchor , Nucleus , Cytoplasm

Background

This gene encodes a member of the regulator of G-protein signaling family. This protein contains a conserved, 120 amino acid motif called the RGS domain and a cysteine-rich region. The protein attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106449_40150_IFA_19011506_118.jpg

GTX106449 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using RGS17(GTX106449) antibody at 1:200 dilution.

GTX106449_40150_WB_B_19011506_615.jpg

GTX106449 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: RGS17 transfected 293T lysates
12% SDS PAGE
GTX106449 diluted at 1:5000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169