GeneTex

  • Country / Location Selection

United States (US)

RGS2 antibody [N1C3-3]

Anti-RGS2 antibody [N1C3-3] used in Western Blot (WB). GTX113349
Anti-RGS2 antibody [N1C3-3] used in Western Blot (WB). GTX113349
Anti-RGS2 antibody [N1C3-3] used in Immunoprecipitation (IP). GTX113349

Cat. No. GTX113349

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RGS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RGS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722875

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulator of G protein signaling 2 , G0S8

Background

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 2 belongs to this family. The protein acts as a mediator of myeloid differentiation and may play a role in leukemogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RGS2 antibody [N1C3-3] used in Western Blot (WB). GTX113349

GTX113349 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS2 antibody [N1C3-3] (GTX113349) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RGS2 antibody [N1C3-3] used in Western Blot (WB). GTX113349

GTX113349 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
12% SDS PAGE
GTX113349 diluted at 1:1000

Anti-RGS2 antibody [N1C3-3] used in Immunoprecipitation (IP). GTX113349

GTX113349 IP Image

Immunoprecipitation of RGS2 protein from U87-MG whole cell extracts using 5 μg of RGS2 antibody [N1C3-3] (GTX113349).
Western blot analysis was performed using RGS2 antibody [N1C3-3] (GTX113349) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

There are currently no references for RGS2 antibody [N1C3-3] (GTX113349). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for RGS2 antibody [N1C3-3] (GTX113349). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169