GeneTex
United States (US)

RGS2 antibody

GTX104944_39904_WB_M_19011506_137.jpg
GTX104944_IFA_19011506_921.jpg
GTX104944_39904_20160518_IHC-P_R_19011506_598.jpg
GTX104944_WB_19011506_697.jpg

Cat No. GTX104944

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

H1299 , Neuro2A

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RGS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

G0S8 antibody, RGS2 antibody, regulator of G-protein signaling 2 antibody, cell growth-inhibiting gene 31 protein antibody, G0/G1 switch regulatory protein 8 antibody, G0 to G1 switch regulatory 8, 24kD antibody, cell growth-inhibiting protein 31 antibody, regulator of G-protein signaling 2, 24kDa antibody

Background

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 2 belongs to this family. The protein acts as a mediator of myeloid differentiation and may play a role in leukemogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104944_39904_WB_M_19011506_137.jpg

GTX104944 WB Image

RGS2 antibody detects RGS2 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
12 % SDS-PAGE
RGS2 antibody (GTX104944) dilution: 1:1000

GTX104944_IFA_19011506_921.jpg

GTX104944 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using RGS2(GTX104944) antibody at 1:200 dilution.

GTX104944_39904_20160518_IHC-P_R_19011506_598.jpg

GTX104944 IHC-P Image

RGS2 antibody detects RGS2 protein at cytoplasm and nucleus in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
RGS2 antibody (GTX104944) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104944_WB_19011506_697.jpg

GTX104944 WB Image

Sample (30μg whole cell lysate)
A:H1299
12% SDS PAGE
GTX104944 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169