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RGS4 antibody [HL1522]

Recombinant antibody KO/KD Orthogonal
Anti-RGS4 antibody [HL1522] used in Western Blot (WB). GTX636996
Anti-RGS4 antibody [HL1522] used in Western Blot (WB). GTX636996
Anti-RGS4 antibody [HL1522] used in Western Blot (WB). GTX636996
Anti-RGS4 antibody [HL1522] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636996
Anti-RGS4 antibody [HL1522] used in IHC (Paraffin sections) (IHC-P). GTX636996
Anti-RGS4 antibody [HL1522] used in IHC (Paraffin sections) (IHC-P). GTX636996

Cat. No. GTX636996

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1522

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RGS4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RGS4. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulator of G protein signaling 4 , RGP4 , SCZD9

Background

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RGS4 antibody [HL1522] used in Western Blot (WB). GTX636996

GTX636996 WB Image

KO/KD

Unboiled wild-type (WT) and RGS4 knockout (KO) SH-SY5Y cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RGS4 antibody [HL1522] used in Western Blot (WB). GTX636996

GTX636996 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-RGS4 antibody [HL1522] used in Western Blot (WB). GTX636996

GTX636996 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RGS4 antibody [HL1522] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636996

GTX636996 ICC/IF Image

RGS4 antibody [HL1522] detects RGS4 protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RGS4 antibody [HL1522] used in IHC (Paraffin sections) (IHC-P). GTX636996

GTX636996 IHC-P Image

RGS4 antibody [HL1522] detects RGS4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RGS4 antibody [HL1522] used in IHC (Paraffin sections) (IHC-P). GTX636996

GTX636996 IHC-P Image

RGS4 antibody [HL1522] detects RGS4 protein at cell membrane and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations