Unboiled wild-type (WT) and RGS4 knockout (KO) SH-SY5Y cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

RGS4 antibody [HL1522] detects RGS4 protein at cell membrane and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RGS4 antibody [HL1522] detects RGS4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RGS4 antibody [HL1522] detects RGS4 protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.