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RHOG antibody

Anti-RHOG antibody used in Western Blot (WB). GTX17227
Anti-RHOG antibody used in IHC (Paraffin sections) (IHC-P). GTX17227

Cat. No. GTX17227

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all RHOG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Human Kidney Tissue Lysate(GTX29995)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RHOG antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOG.The immunogen is located within amino acids 40 - 90 of RHOG.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ras homolog family member G , ARHG

Cellular Localization

Cell membrane

Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein facilitates translocation of a functional guanine nucleotide exchange factor (GEF) complex from the cytoplasm to the plasma membrane where ras-related C3 botulinum toxin substrate 1 is activated to promote lamellipodium formation and cell migration. Two related pseudogene have been identified on chromosomes 20 and X. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-RHOG antibody used in Western Blot (WB). GTX17227

GTX17227 WB Image

WB analysis of human kidney tissue lysate using GTX17227 RHOG antibody.
Working concentration : 1 μg/ml

Anti-RHOG antibody used in IHC (Paraffin sections) (IHC-P). GTX17227

GTX17227 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX17227 RHOG antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399