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RICTOR antibody [7B3]

Anti-RICTOR antibody [7B3] used in IHC (Paraffin sections) (IHC-P). GTX82789
Anti-RICTOR antibody [7B3] used in Flow cytometry (FACS). GTX82789
Anti-RICTOR antibody [7B3] used in Western Blot (WB). GTX82789
Anti-RICTOR antibody [7B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82789

Cat. No. GTX82789

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7B3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($399)
See all RICTOR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

192 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human RICTOR expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RPTOR independent companion of MTOR complex 2 , AVO3 , PIA , hAVO3

Background

RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-RICTOR antibody [7B3] used in IHC (Paraffin sections) (IHC-P). GTX82789

GTX82789 IHC-P Image

IHC-P analysis of thyroid gland tissue (left) and human breast carcinoma (right) using GTX82789 RICTOR antibody [7B3].

Anti-RICTOR antibody [7B3] used in Flow cytometry (FACS). GTX82789

GTX82789 FACS Image

FACS analysis of HeLa cells using GTX82789 RICTOR antibody [7B3].
Green : RICTOR
Purple : negative control

Anti-RICTOR antibody [7B3] used in Western Blot (WB). GTX82789

GTX82789 WB Image

WB analysis of HeLa (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate using GTX82789 RICTOR antibody [7B3].

Anti-RICTOR antibody [7B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82789

GTX82789 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX82789 RICTOR antibody [7B3].
Green : RICTOR
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399