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RICTOR antibody [C3], C-term

Anti-RICTOR antibody [C3], C-term used in Immunohistochemistry (IHC). GTX104617
Anti-RICTOR antibody [C3], C-term used in Western Blot (WB). GTX104617
Anti-RICTOR antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104617

Cat No. GTX104617

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

192 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RICTOR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951713

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RPTOR independent companion of MTOR complex 2 , AVO3 , PIA , hAVO3

Background

RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-RICTOR antibody [C3], C-term used in Immunohistochemistry (IHC). GTX104617

GTX104617 IHC Image

Staining of Human Kidney cancer tissue sections using anti-Rictor antibody (GTX104617).

Anti-RICTOR antibody [C3], C-term used in Western Blot (WB). GTX104617

GTX104617 WB Image

Sample(30 ug whole cell lysate)
A: A431 (GTX27909)
5% SDS PAGE
GTX104617 diluted at 1:1000

Anti-RICTOR antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104617

GTX104617 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RICTOR(GTX104617) antibody at 1:200 dilution.

REFERENCE

REVIEW

RICTOR antibody [C3], C-term Cat No. GTX104617

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018.PMID: 29712898
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169