GeneTex
United States (US)

RICTOR antibody [C3], C-term

Genetex  Rictor 617S 400X 1in 50000 human kidney cancer 2.2011_19011720_431.jpg
GTX104617_WB_19011506_919.jpg
GTX104617_IFA_19011506_870.jpg

Cat No. GTX104617

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

192 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RICTOR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686B11164 antibody, KIAA1999 antibody, MGC39830 antibody, PIA antibody, mAVO3 antibody, RICTOR antibody, hAVO3 antibody, AVO3 homolog antibody, pianissimo antibody, rapamycin-insensitive companion of mTOR antibody, TORC2-specific protein AVO3 antibody, "RPTOR independent companion of MTOR, complex 2 antibody"

Background

RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Genetex  Rictor 617S 400X 1in 50000 human kidney cancer 2.2011_19011720_431.jpg

GTX104617 IHC Image

Staining of Human Kidney cancer tissue sections using anti-Rictor antibody (GTX104617).

GTX104617_WB_19011506_919.jpg

GTX104617 WB Image

Sample(30 ug whole cell lysate)
A: A431 (GTX27909)
5% SDS PAGE
GTX104617 diluted at 1:1000

GTX104617_IFA_19011506_870.jpg

GTX104617 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RICTOR(GTX104617) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169