Rif1 antibody detects Rif1 protein at cytoplasm and nucleus in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Rif1 antibody (GTX131889) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rif1 antibody detects Rif1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rif1 antibody (GTX131889) diluted at 1:2000.

Immunoprecipitation of Rif1 protein from HeLa whole cell extracts using 5 μg of Rif1 antibody (GTX131889).
Western blot analysis was performed using Rif1 antibody (GTX131889).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Rif1 antibody detects Rif1 protein at cytoplasm and nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Rif1 antibody (GTX131889) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rif1 antibody detects Rif1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rif1 protein stained by Rif1 antibody (GTX131889) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RIF1 antibody (GTX131889) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.