GeneTex
  • Country / Location Selection

United States (US)

RIF1 antibody

Anti-RIF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131889
Anti-RIF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131889
Anti-RIF1 antibody used in Immunoprecipitation (IP). GTX131889
Anti-RIF1 antibody used in Western Blot (WB). GTX131889
Anti-RIF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131889

Cat No. GTX131889

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

274 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus of human RIF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

replication timing regulatory factor 1

Cellular Localization

Nucleus , Telomere

Background

This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES
Anti-RIF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131889

GTX131889 ICC/IF Image

Rif1 antibody detects Rif1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rif1 protein stained by Rif1 antibody (GTX131889) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RIF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131889

GTX131889 IHC-P Image

Rif1 antibody detects Rif1 protein at cytoplasm and nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Rif1 antibody (GTX131889) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RIF1 antibody used in Immunoprecipitation (IP). GTX131889

GTX131889 IP Image

Immunoprecipitation of Rif1 protein from HeLa whole cell extracts using 5 μg of Rif1 antibody (GTX131889).
Western blot analysis was performed using Rif1 antibody (GTX131889).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-RIF1 antibody used in Western Blot (WB). GTX131889

GTX131889 WB Image

Rif1 antibody detects Rif1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rif1 antibody (GTX131889) diluted at 1:2000.

Anti-RIF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131889

GTX131889 IHC-P Image

Rif1 antibody detects Rif1 protein at cytoplasm and nucleus in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Rif1 antibody (GTX131889) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
There are currently no references for RIF1 antibody (GTX131889). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for RIF1 antibody (GTX131889). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169