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RIN1 antibody

Anti-RIN1 antibody used in Western Blot (WB). GTX131627

Cat. No. GTX131627

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RIN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

293T , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RIN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886508

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ras and Rab interactor 1

Cellular Localization

Cytoplasm , Membrane , cytoskeleton

Background

Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis.

Database

Research Area

DATA IMAGES

Anti-RIN1 antibody used in Western Blot (WB). GTX131627

GTX131627 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RIN1 antibody (GTX131627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

RIN1 antibody Cat. No. GTX131627

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-RIN1 antibody used in Western Blot (WB). GTX131627
Date : Anonymous submitted on 25-Apr-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : U937 cells
Amount used : 30μg
Blocking : PVDF Blocking reagent (TOYOBO), 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, > 12Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169