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RIN2 antibody

Anti-RIN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117153
Anti-RIN2 antibody used in Western Blot (WB). GTX117153
Anti-RIN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117153
Anti-RIN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117153

Cat No. GTX117153

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RIN2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163802

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ras and Rab interactor 2 , MACS , RASSF4

Cellular Localization

Cytoplasm

Background

The RAB5 protein is a small GTPase involved in membrane trafficking in the early endocytic pathway. The protein encoded by this gene binds the GTP-bound form of the RAB5 protein preferentially over the GDP-bound form, and functions as a guanine nucleotide exchange factor for RAB5. The encoded protein is found primarily as a tetramer in the cytoplasm and does not bind other members of the RAB family. [provided by RefSeq]

Database

DATA IMAGES

Anti-RIN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117153

GTX117153 IHC-P Image

RIN2 antibody detects RIN2 protein at cytosol on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
RIN2 antibody (GTX117153) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RIN2 antibody used in Western Blot (WB). GTX117153

GTX117153 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
5% SDS PAGE
GTX117153 diluted at 1:1000

Anti-RIN2 antibody used in IHC (Paraffin sections) (IHC-P). GTX117153

GTX117153 IHC-P Image

RIN2 antibody detects RIN2 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
RIN2 antibody (GTX117153) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RIN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117153

GTX117153 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RIN2(GTX117153) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169