GeneTex
United States (US)

RING1 antibody [N1N3]

GTX101671_40275_IHC_19011506_818.jpg
GTX101671_WB_19011506_882.jpg

Cat No. GTX101671

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RING1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RING1A antibody, RNF1 antibody, RING1 antibody, really interesting new gene 1 protein antibody, polycomb complex protein RING1 antibody, E3 ubiquitin-protein ligase RING1 antibody, ring finger protein 1 antibody

Background

This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq]

Database

DATA IMAGES
GTX101671_40275_IHC_19011506_818.jpg

GTX101671 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using RING1(GTX101671) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101671_WB_19011506_882.jpg

GTX101671 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX101671 diluted at 1:3000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169