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RIOK3 antibody

Anti-RIOK3 antibody used in Western Blot (WB). GTX107514
Anti-RIOK3 antibody used in Western Blot (WB). GTX107514
Anti-RIOK3 antibody used in Western Blot (WB). GTX107514
Anti-RIOK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX107514

Cat. No. GTX107514

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RIOK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

293T , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RIOK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174804

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RIO kinase 3 , SUDD

Background

This gene was identified by the similarity of its product to the Aspergillus nidulans SUDD protein, an extragenic suppressor of the heat-sensitive bimD6 mutation that fails to attach properly to the spindle microtubules at a restrictive temperature. The specific function of this gene has not yet been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RIOK3 antibody used in Western Blot (WB). GTX107514

GTX107514 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RIOK3 antibody (GTX107514) diluted at 1:1000.

Anti-RIOK3 antibody used in Western Blot (WB). GTX107514

GTX107514 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: HepG2
7.5% SDS PAGE
GTX107514 diluted at 1:2000

Anti-RIOK3 antibody used in Western Blot (WB). GTX107514

GTX107514 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RIOK3 antibody (GTX107514) diluted at 1:1000.

Anti-RIOK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX107514

GTX107514 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RIOK3(GTX107514) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169