GeneTex
United States (US)

RIOK3 antibody

GTX107514_40744_IHC_19011506_227.jpg
GTX107514_40744_WB_19011506_568.jpg
GTX107514_40744_20160825_WB_M_19011506_640.jpg
GTX107514_40744_20160825_WB_R_19011506_504.jpg

Cat No. GTX107514

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

293T , HepG2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RIOK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rio Kinase 3,Sudd,Riok3

Background

This gene was identified by the similarity of its product to the Aspergillus nidulans SUDD protein, an extragenic suppressor of the heat-sensitive bimD6 mutation that fails to attach properly to the spindle microtubules at a restrictive temperature. The specific function of this gene has not yet been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107514_40744_IHC_19011506_227.jpg

GTX107514 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RIOK3(GTX107514) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107514_40744_WB_19011506_568.jpg

GTX107514 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: HepG2
7.5% SDS PAGE
GTX107514 diluted at 1:2000

GTX107514_40744_20160825_WB_M_19011506_640.jpg

GTX107514 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RIOK3 antibody (GTX107514) diluted at 1:1000.

GTX107514_40744_20160825_WB_R_19011506_504.jpg

GTX107514 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RIOK3 antibody (GTX107514) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169