GeneTex
United States (US)

RIP / Rab antibody

GTX114963_40471_IFA_18121410_714.jpg
GTX114963_40471_IHC_18121410_544.jpg
GTX114963_40471_WB_18121410_769.jpg

Cat No. GTX114963

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RIP / Rab. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686I15205 antibody, HRB antibody, MGC116938 antibody, MGC116940 antibody, RAB antibody, RIP antibody, AGFG1 antibody, HIV-1 Rev-binding protein antibody, nucleoporin-like protein RIP antibody, HIV-1 Rev binding protein antibody, arf-GAP domain and FG repeats-containing protein 1 antibody, "hRIP, Rev interacting protein antibody", "Rab, Rev/Rex activation domain-binding protein antibody", rev/Rex activation domain-binding protein antibody, rev-interacting protein antibody, ArfGAP with FG repeats 1 antibody

Cellular Localization

Nucleus , Cytoplasmic vesicle

Background

The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX114963_40471_IFA_18121410_714.jpg

GTX114963 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RIP/Rab(GTX114963) antibody at 1:500 dilution.

GTX114963_40471_IHC_18121410_544.jpg

GTX114963 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RIP/Rab(GTX114963) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114963_40471_WB_18121410_769.jpg

GTX114963 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX114963 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169