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RIP antibody [GT1168]

Recombinant antibody
Anti-RIP antibody [GT1168] used in Western Blot (WB). GTX09128
Anti-RIP antibody [GT1168] used in Western Blot (WB). GTX09128
Anti-RIP antibody [GT1168] used in Immunoprecipitation (IP). GTX09128

Cat. No. GTX09128

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1168

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399)
See all RIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan RIP

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

receptor interacting serine/threonine kinase 1 , IMD57 , RIP , RIP-1 , RIP1

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aμg 2017]

Database

Research Area

DATA IMAGES

Anti-RIP antibody [GT1168] used in Western Blot (WB). GTX09128

GTX09128 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP antibody [GT1168] (GTX09128) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RIP antibody [GT1168] used in Western Blot (WB). GTX09128

GTX09128 WB Image

WB analysis of Raji and HeLa whole cell lysates using GTX09128 RIP antibody [GT1168].
Dilution : 1:1000
Loading : 25μg

Anti-RIP antibody [GT1168] used in Immunoprecipitation (IP). GTX09128

GTX09128 IP Image

IP analysis of HeLa cell lysate using GTX09128 RIP antibody [GT1168].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399