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RIP antibody [HL2250]

Recombinant antibody Orthogonal
Anti-RIP antibody [HL2250] used in Western Blot (WB). GTX638299
Anti-RIP antibody [HL2250] used in Western Blot (WB). GTX638299
Anti-RIP antibody [HL2250] used in IHC (Paraffin sections) (IHC-P). GTX638299
Anti-RIP antibody [HL2250] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638299

Cat. No. GTX638299

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2250

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human RIP.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

receptor interacting serine/threonine kinase 1 , IMD57 , RIP , RIP-1 , RIP1

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-RIP antibody [HL2250] used in Western Blot (WB). GTX638299

GTX638299 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RIP antibody [HL2250] (GTX638299) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RIP antibody [HL2250] used in Western Blot (WB). GTX638299

GTX638299 WB Image

Orthogonal

Untreated (–) and treated (+) NCI-H929 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RIP antibody [HL2250] (GTX638299) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RIP antibody [HL2250] used in IHC (Paraffin sections) (IHC-P). GTX638299

GTX638299 IHC-P Image

RIP antibody [HL2250] detects RIP protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
RIP stained by RIP antibody [HL2250] (GTX638299) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RIP antibody [HL2250] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638299

GTX638299 ICC/IF Image

RIP antibody [HL2250] detects RIP protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RIP stained by RIP antibody [HL2250] (GTX638299) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations