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RIP antibody

Anti-RIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111074
Anti-RIP antibody used in Western Blot (WB). GTX111074

Cat. No. GTX111074

Reference 7
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

Molt-4

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RIP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951716

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

receptor interacting serine/threonine kinase 1 , IMD57 , RIP , RIP-1 , RIP1

Background

Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.

Database

Research Area

DATA IMAGES

Anti-RIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111074

GTX111074 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using RIP(GTX111074) antibody at 1:100 dilution.

Anti-RIP antibody used in Western Blot (WB). GTX111074

GTX111074 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX111074 diluted at 1:5000

REFERENCE

Application Reference

Lanzillotta C et al. Prog Neurobiol 2020; : 101892 Chronic PERK induction promotes Alzheimer-like neuropathology in Down syndrome: Insights for therapeutic intervention.
Application : WB
Reactivity : Human
Chen YH et al. Cancer Manag Res 2020; 12 : 4645-4665
Application : WB
Reactivity : Human
Saemisch M et al. Am J Physiol Cell Physiol 2019; 316 (2) : C210-C222 Subendothelial matrix components influence endothelial cell apoptosis in vitro.
Application : WB
Reactivity : Human
Saemisch M et al. J Tissue Eng Regen Med 2017; (Epub) 3D matrix embedding inhibits cycloheximide-mediated sensitization to TNF-alpha-induced apoptosis of human endothelial cells.
Application : WB
Tsui KH et al. Reproduction 2017; 154 (2) : 101-110 DHEA protects mitochondria against dual modes of apoptosis and necroptosis in human granulosa HO23 cells.
Application : WB
Reactivity : Human
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REVIEW

RIP antibody Cat. No. GTX111074

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-RIP antibody used in Western Blot (WB). GTX111074
Date : Anonymous submitted on 22-Nov-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : Jurkat cells
Amount used : 60μg
Blocking : 5% milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169